Dissemination of IT for the Promotion of Materials ScienceDoITPoMS Teaching and Learning Packages

Optical Microscopy and Specimen Preparation

Answer to Q7

 
  1. If a graticule is observed under the 10x lens of a microscope so that the diameter of the field of view is from 150 µm to 450 µm on the graticule, what is the width of one lamella when it takes 15 lamellae to fill the field of view when viewed under the 50x lens.
      Answer = 4 µm

    Worked Answer

    The field of view under the 10x lens is 450 µm - 150 µm = 300 µm.

    So the field of view under the 50x lens is 300 µm/5 = 60 µm.

    If 15 lamellae fill the field of view, then each lamella must be 60 µm/15 = 4 µm across.

     
     
     
     
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